Research Technologist
GENERAL SUMMARY/ OVERVIEW STATEMENT:
Dr. Edlow's basic and translational research in the Vincent Center for Reproductive Biology (VCRB) focuses on neurodevelopmental programming effects of maternal obesity and maternal high-fat and other dietary conditions in pregnancy. The work is primarily with a mouse model of maternal diet-induced obesity and includes both embryonic brain work and work with juvenile and adult offspring brain assays and neurobehavioral testing.
The Laboratory Research Technologist reports directly to the Principal Investigator, Director, and/or managers of the laboratory and is expected to work independently on assigned research with minimal guidance from a higher level staff member or Principal Investigator. The Laboratory Research Technologist may also serve as team leader to other lab assistants and Research Technicians I and II, if applicable. The ideal Technologist is expected to have extensive experience in mouse models and handling, in particular brain retrieval/dissection, preservation for RNA and immunohistochemical analyses, and brain sectioning and tissue staining. The Technologist is expected to lead a research project to study the neurodevelopmental programming effects of maternal obesity and maternal dietary manipulations in pregnancy and lactation, primarily using mouse and cellular models in a biomedical research laboratory. There may also be limited human sample processing and analyses in the future. With broad guidance from the supervisor, the Technologist is expected to independently design and perform experiments to accomplish laboratory research goals. In addition, s/he will be responsible for documenting, analyzing, and reporting the results derived from mouse models and/or patient samples. The Technologist will assist in the preparation of manuscripts for publication. The individual will also assist with gathering information needed for grant preparation and reporting and must be proficient in basic computer skills including word-processing, database and spreadsheet management, email and internet.
PRINCIPAL DUTIES AND
Responsibilities:
Primary responsibility of this role will be performing experiments in a biomedical research laboratory for NIH-funded research, studying the effects of maternal obesity on embryonic brain development and offspring brain and behavior. The position requires a team-based approach between the PI and Technologist where the Technologist's input into experimental designs and protocols, and recommendations for innovation, will be highly valued.
Specific skills that are sought include:
Experience with mouse/rodent models, including colony management, record-keeping, timed matings, behavioral studies, surgery.
Experience with brain retrieval and dissection in juvenile and adult mice, ideally including brain perfusion. Experience with embryonic dissection and handling, ideally including brain, preferred.
Comprehensive knowledge of and experience with a variety of lab techniques, including DNA, RNA and protein extraction, quantitative PCR experiment design and interpretation, western blotting, ELISA.
Experience with tissue sectioning on the cryostat and/or microtome, ideally of brain.
Experience with basic histology, immunohistochemistry and microscopy techniques.
Skill in collection, organization, management, and analysis of complex data sets including record-keeping for large mouse colonies and neurobehavioral test results, among others.
Skill in preparation of tables, charts, graphs, summaries, and other materials for effective presentation and publication of experimental results.
Ability to analyze the cause and significance of unexpected experimental results and to modify or redesign techniques and methods as necessary.
The primary responsibilities for this experienced research position are as follows:
Lead a research project to study the effects of maternal obesity and maternal nutrition on fetal and offspring brain development.
S/he is expected to design and conduct experiments independently in the laboratory with general guidance from the supervisor, and participate in the design of complex experiments.
Develop new experimental protocols, execute protocols of non-routine experiments, troubleshoot experimental problems and provide alternative approaches.
Train junior researchers and technicians in laboratory methods and techniques routinely used in this project, as outlined above under specific skills sought. Oversee trainee record-keeping and experimental conduct.
Maintain quality control of all research efforts.
Maintain detailed records of all experiments and protocols in lab notebook form on a day-to-day basis, as well as experimental results in a tabular format (e.g. Excel) to facilitate easy analysis.
Independently collect and frequently analyze experimental results using appropriate statistical analysis. Judge the validity and reliability of experimental data. Summarize research results, and prepare written progress reports.
Present research results at regional, national or international scientific conferences.
Coordinate and collaborate with investigators in other laboratories to ensure success of ongoing or new projects. Work with scientists across a broad range of backgrounds.
Prepare data for grants and manuscripts.
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
The Technologist should have a strong background in molecular biology, biochemistry, genetics, and neuroscience (ideally neurodevelopment and/or neuroanatomy). The Technologist should understand theories behind assays and procedures, and have the ability to identify problems and develop solutions.
The Technologist should be able to work independently and display initiative to introduce new methodologies to research study.
The Technologist should have comprehensive knowledge of experimental animal care and handling, especially handling and breeding of mice, and surgery/tissue retrieval from embryos, juvenile and adult offspring generated in the colony.
Ability to prepare IACUC and IRB protocols and maintain compliance with required periodic documentation.
Ability to prioritize tasks and meet deadlines.
Sound interpersonal skills and the ability to train and supervise junior laboratory members.
Excellent oral and written communication skills.
Computer competency is required.
Knowledge of Microsoft Office and statistical analytical tools is required. Experience with Graphpad Prism or SPSS/SAS is preferred.
Must be resourceful, self motivated, highly organized, have good time management skills, work cooperatively with others and be willing to learn new techniques
LICENSES, CERTIFICATIONS, and/or REGISTRATIONS (if applicable):
N/A
EDUCATION:
BS/BA degree with significant formal biomedical research training is required. Higher degree including master's degree or PhD is preferred but not required, depending on experience.
Experience:
At least 3 and preferably more years of experience and/or training related to neuroscience research is required.
3 or more years' experience working with rodent models is required.
Extensive experience in small animal husbandry and surgery are highly preferred.
Previous laboratory experience in molecular biology and histological studies is required, cell culture experience is preferred.
SUPERVISORY RESPONSIBILITY (if applicable):
No direct reports. The Technologist will be expected to provide necessary research support for other researchers and assist the training of students, postdocs and fellows.
Laboratory environment.
At least 3 and preferably more years of experience and/or training related to neuroscience research is required.
3 or more years' experience working with rodent models is required.
Extensive experience in small animal husbandry and surgery are highly preferred.
Previous laboratory experience in molecular biology and histological studies is required, cell culture experience is preferred.
. Apply now!Estimated Salary: $20 to $28 per hour based on qualifications.
Dr. Edlow's basic and translational research in the Vincent Center for Reproductive Biology (VCRB) focuses on neurodevelopmental programming effects of maternal obesity and maternal high-fat and other dietary conditions in pregnancy. The work is primarily with a mouse model of maternal diet-induced obesity and includes both embryonic brain work and work with juvenile and adult offspring brain assays and neurobehavioral testing.
The Laboratory Research Technologist reports directly to the Principal Investigator, Director, and/or managers of the laboratory and is expected to work independently on assigned research with minimal guidance from a higher level staff member or Principal Investigator. The Laboratory Research Technologist may also serve as team leader to other lab assistants and Research Technicians I and II, if applicable. The ideal Technologist is expected to have extensive experience in mouse models and handling, in particular brain retrieval/dissection, preservation for RNA and immunohistochemical analyses, and brain sectioning and tissue staining. The Technologist is expected to lead a research project to study the neurodevelopmental programming effects of maternal obesity and maternal dietary manipulations in pregnancy and lactation, primarily using mouse and cellular models in a biomedical research laboratory. There may also be limited human sample processing and analyses in the future. With broad guidance from the supervisor, the Technologist is expected to independently design and perform experiments to accomplish laboratory research goals. In addition, s/he will be responsible for documenting, analyzing, and reporting the results derived from mouse models and/or patient samples. The Technologist will assist in the preparation of manuscripts for publication. The individual will also assist with gathering information needed for grant preparation and reporting and must be proficient in basic computer skills including word-processing, database and spreadsheet management, email and internet.
PRINCIPAL DUTIES AND
Responsibilities:
Primary responsibility of this role will be performing experiments in a biomedical research laboratory for NIH-funded research, studying the effects of maternal obesity on embryonic brain development and offspring brain and behavior. The position requires a team-based approach between the PI and Technologist where the Technologist's input into experimental designs and protocols, and recommendations for innovation, will be highly valued.
Specific skills that are sought include:
Experience with mouse/rodent models, including colony management, record-keeping, timed matings, behavioral studies, surgery.
Experience with brain retrieval and dissection in juvenile and adult mice, ideally including brain perfusion. Experience with embryonic dissection and handling, ideally including brain, preferred.
Comprehensive knowledge of and experience with a variety of lab techniques, including DNA, RNA and protein extraction, quantitative PCR experiment design and interpretation, western blotting, ELISA.
Experience with tissue sectioning on the cryostat and/or microtome, ideally of brain.
Experience with basic histology, immunohistochemistry and microscopy techniques.
Skill in collection, organization, management, and analysis of complex data sets including record-keeping for large mouse colonies and neurobehavioral test results, among others.
Skill in preparation of tables, charts, graphs, summaries, and other materials for effective presentation and publication of experimental results.
Ability to analyze the cause and significance of unexpected experimental results and to modify or redesign techniques and methods as necessary.
The primary responsibilities for this experienced research position are as follows:
Lead a research project to study the effects of maternal obesity and maternal nutrition on fetal and offspring brain development.
S/he is expected to design and conduct experiments independently in the laboratory with general guidance from the supervisor, and participate in the design of complex experiments.
Develop new experimental protocols, execute protocols of non-routine experiments, troubleshoot experimental problems and provide alternative approaches.
Train junior researchers and technicians in laboratory methods and techniques routinely used in this project, as outlined above under specific skills sought. Oversee trainee record-keeping and experimental conduct.
Maintain quality control of all research efforts.
Maintain detailed records of all experiments and protocols in lab notebook form on a day-to-day basis, as well as experimental results in a tabular format (e.g. Excel) to facilitate easy analysis.
Independently collect and frequently analyze experimental results using appropriate statistical analysis. Judge the validity and reliability of experimental data. Summarize research results, and prepare written progress reports.
Present research results at regional, national or international scientific conferences.
Coordinate and collaborate with investigators in other laboratories to ensure success of ongoing or new projects. Work with scientists across a broad range of backgrounds.
Prepare data for grants and manuscripts.
SKILLS/ABILITIES/COMPETENCIES REQUIRED:
The Technologist should have a strong background in molecular biology, biochemistry, genetics, and neuroscience (ideally neurodevelopment and/or neuroanatomy). The Technologist should understand theories behind assays and procedures, and have the ability to identify problems and develop solutions.
The Technologist should be able to work independently and display initiative to introduce new methodologies to research study.
The Technologist should have comprehensive knowledge of experimental animal care and handling, especially handling and breeding of mice, and surgery/tissue retrieval from embryos, juvenile and adult offspring generated in the colony.
Ability to prepare IACUC and IRB protocols and maintain compliance with required periodic documentation.
Ability to prioritize tasks and meet deadlines.
Sound interpersonal skills and the ability to train and supervise junior laboratory members.
Excellent oral and written communication skills.
Computer competency is required.
Knowledge of Microsoft Office and statistical analytical tools is required. Experience with Graphpad Prism or SPSS/SAS is preferred.
Must be resourceful, self motivated, highly organized, have good time management skills, work cooperatively with others and be willing to learn new techniques
LICENSES, CERTIFICATIONS, and/or REGISTRATIONS (if applicable):
N/A
EDUCATION:
BS/BA degree with significant formal biomedical research training is required. Higher degree including master's degree or PhD is preferred but not required, depending on experience.
Experience:
At least 3 and preferably more years of experience and/or training related to neuroscience research is required.
3 or more years' experience working with rodent models is required.
Extensive experience in small animal husbandry and surgery are highly preferred.
Previous laboratory experience in molecular biology and histological studies is required, cell culture experience is preferred.
SUPERVISORY RESPONSIBILITY (if applicable):
No direct reports. The Technologist will be expected to provide necessary research support for other researchers and assist the training of students, postdocs and fellows.
Laboratory environment.
At least 3 and preferably more years of experience and/or training related to neuroscience research is required.
3 or more years' experience working with rodent models is required.
Extensive experience in small animal husbandry and surgery are highly preferred.
Previous laboratory experience in molecular biology and histological studies is required, cell culture experience is preferred.
. Apply now!Estimated Salary: $20 to $28 per hour based on qualifications.
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