ESC


Sylvia Eunsun Cho, MA



580 Commonwealth Avenue #602 • Boston, MA 02215


(617) 510-3899 • sylviacho605@hotmail.com


Scientific Researcher


Highly analytic and solutions-oriented professional with 6+ years of experience in conducting extensive scientific research in the areas of Biochemistry, Molecular and Cell Biology, Microbiology and Genetics.


Spearheaded and completed various research projects that required accurate data collection, experimental designing, activity assay, protein purification, kinetics measuring, and product analysis. Led the improvement process of a laboratory by executing procedures, researching and developing equipment and re-designing inventory layout. Instrumental in the success of the product Biosimilar (a generic form of bio-medicine) meeting EMEA and FDA standards for clinical trials and market sale by providing innovative solutions on technical and strategic issues. Provided quality instruction for up to 20 students consecutively at the undergraduate level within a classroom and laboratory setting. Technical and language expertise includes: 























Computer:



Microsoft Office Suite, Macintosh and Windows Operating systems, Internet Explorer, PubMed, Google Scholar, SciFinder, EndNote, SigmaPlot, GraphPad Prism, R (statistical analysis system), Chemdraw, SnapGene



Lab:



Experimental Design, Protein expression and purification (Strep-tag, His-tag, Flag-tag, Ammonium Sulfate precipitation, Ion exchange, and size-exclusion column), Pull-down assay, Protein activity assay and analysis, SDS-PAGE, Gel Electrophoresis, Western Blot, Transformation (electroporation and heat shock), Transfection, Polymerase Chain Reaction (PCR), DNA extraction and sequencing, Cloning, Genome editing, Gibson method, Cell culture, Fermenter, Microbiology,  Lyophilizer, Ultrafiltration, Bradford assay, Centrifuge, ImageLab (Bio-rad), Microplate reader, UV-Vis spectrum, Anaerobic  glove box (Coy chamber), NMR, EPR, Stopped-flow, GC or LC/MS, IR, CD, HPLC



Others:



Data Analysis, Organization, Project Management, Teamwork, Public Speaking, GCP, GMP,



Language:



Korean, English (native fluency in both)




 














Experience Highlights



 



 




Boston University, Boston, MA


Teaching Fellow (Chemistry & Biology Departments), 9/2012 – 8/2016


An integral part in teaching up to 20 students in undergraduate chemistry laboratory courses each semester (3 sections assigned). Discussed topics which included: General Chemistry, Biochemistry, Organic Chemistry, Principles of Organic and Biochemistry, Human Physiology, and General Chemistry for School of Engineering. Introduced concepts to students that were vital to their success in each laboratory section. Held weekly office hours to assist students with questions concerning lab report and concepts.


 


Graduate Student Researcher/Undergraduate Research Assistant, 1/2009 – 8/2016


Operated as Lab Manager and Safety Coordinator for two years, where primary responsibilities were to ensure chemical and supplies were ordered, laboratory inventory were managed accurately, equipment maintenance was scheduled accordingly and work conditions remained safe according to standards. Educated undergraduate students on general laboratory skills to prepare them for senior level project assisting.


 


Selected Contributions:



  • Introduced and researched Malbrancheamide, a project associated with the noble natural product from fungi. Key functions were to collect and analyze primary data, create experimental designs, and research the proposed biosynthetic pathway to ensure its accuracy.

  • Focused on the Rieske-type demethylase (Stc2) project. Tasks included executing cell growth, protein purification and characterization, activity assay (with various substrates), and biophysics experiments. Participated in preparing two scientific paper publication.

  • Led the MEP pathway project with a post-doctorate researcher, and generated deuterium characterized as MEcPP via biosynthesis. Accountable for protein purification, activity assay, product purification and analysis and kinetics measurement; thesis was successfully defended.


 


Celltrion, Incheon, South Korea


Intern, 5/2009 – 8/2009


An essential part in developing biosimilar (generic form of bio-medicine) through advisement on technical and strategic issues. Contributed to preparing regulatory file applications and supporting documentations to KFDA for clinical trials. Organized regulations about biosimilar from EMEA and FDA to meet qualifications for clinical trials and sale.


 














Education & Credentials



 



 




Boston University, Boston, MA


Master of Arts, Chemistry, 9/2016 (Expected)


Relevant Coursework: Research in Chemistry, Epigenetics, Natural Products, Chemical Biology, Specific Topics in Biochemistry, Current Topics in Organic Chemistry, Research Methods - (GPA: 3.68/4.00)


 


Master of Arts, Molecular, Cell Biology and Biochemistry, 9/2014


Relevant Coursework: Cell Biology, Molecular Biology, Progress in Cell and Molecular Biology, Biostatistics, Biochemistry, Research in Biology - (GPA: 3.58/4.00)


 


Bachelor of Arts, Biochemistry and Molecular Biology & Psychology (double major), 9/2011


Relevant Coursework: Biochemistry and Lab, Molecular Biology, Genetics, Organic Chemistry, Research in Chemistry and Biology, Physiology, Physical Biochemistry, Statistics, Cognitive Neuroscience, Abnormal Psychology, Family Psychology, Experimental Psychology - (GPA: 3.20/4.00)

Publications


─     Roessler, C.G.; Agarwal, R.; Allaire, M.; Alonso-Mori, R.; Andi, B.; Bachega, J.F.; Bommer, M.; Brewster, A.S.; Browne, M.C.; Chatterjee, R. and Cho, E., Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography. Structure 201624 (4), 631-640.


─     Daughtry, K. D.; Xiao, Y.; Stoner-Ma, D.; Cho, E.; Orville, A. M.; Liu, P.; Allen, K. N., Quaternary Ammonium Oxidative Demethylation: X-ray Crystallographic, Resonance Raman, and UV-Visible Spectroscopic Analysis of a Rieske-Type Demethylase. Journal of the American Chemical Society 2012, 134 (5), 2823-2834.


 

  • ID#: 120349
  • Location: Boston, MA , 02215

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